Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources
نویسندگان
چکیده
منابع مشابه
Exoproteome profiles of Clostridium cellulovorans grown on various carbon sources.
The cellulosome is a complex of cellulosomal proteins bound to scaffolding proteins. This complex is considered the most efficient system for cellulose degradation. Clostridium cellulovorans, which is known to produce cellulosomes, changes the composition of its cellulosomes depending on the growth substrates. However, studies have investigated only cellulosomal proteins; profile changes in non...
متن کاملSynergistic interaction of Clostridium cellulovorans cellulosomal cellulases and HbpA.
Clostridium cellulovorans, an anaerobic bacterium, produces a small nonenzymatic protein called HbpA, which has a surface layer homology domain and a type I cohesin domain similar to those found in the cellulosomal scaffolding protein CbpA. In this study, we demonstrated that HbpA could bind to cell wall fragments from C. cellulovorans and insoluble polysaccharides and form a complex with cellu...
متن کاملTranscription of Clostridium cellulovorans cellulosomal cellulase and hemicellulase genes.
Transcription of the cellulosomal cellulase/hemicellulase genes of Clostridium cellulovorans has been investigated by Northern blot, reverse transcriptase PCR (RT-PCR), primer extension, and S1 nuclease analysis. Northern hybridizations revealed that the cellulosomal cbpA gene cluster is transcribed as polycistronic mRNAs of 8 and 12 kb. The 8-kb mRNA coded for cbpA and exgS, and the 12-kb mRNA...
متن کاملThe engL gene cluster of Clostridium cellulovorans contains a gene for cellulosomal manA.
A five-gene cluster around the gene in Clostridium cellulovorans that encodes endoglucanase EngL, which is involved in plant cell wall degradation, has been cloned and sequenced. As a result, a mannanase gene, manA, has been found downstream of engL. The manA gene consists of an open reading frame with 1,275 nucleotides encoding a protein with 425 amino acids and a molecular weight of 47, 156. ...
متن کاملRegulation of expression of cellulosomal cellulase and hemicellulase genes in Clostridium cellulovorans.
The regulation of expression of the genes encoding the cellulases and hemicellulases of Clostridium cellulovorans was studied at the mRNA level with cells grown under various culture conditions. A basic pattern of gene expression and of relative expression levels was obtained from cells grown in media containing poly-, di- or monomeric sugars. The cellulase (cbpA and engE) and hemicellulase (xy...
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ژورنال
عنوان ژورنال: AMB Express
سال: 2012
ISSN: 2191-0855
DOI: 10.1186/2191-0855-2-37